I am trying to plot an MSA tree for my nucleotide sequences. I used toparslan's script. It worked on other files but with the current file, it won't work. Error on distance information? How do I fix this?
> [1] "DNAbin"
dnbin<-dist.dna(nbin, model = "K80") #computing distance by ape package with K80 model derived by Kimura (1980)
tree<-njs(dnbin)
**Error in njs(dnbin) :
distance information insufficient to construct a tree, cannot calculate agglomeration criterion**
Help would be appreciated!
nbinmat<-as.matrix(labels(nbin)) #extraction of the sample names
nbin
class(nbin)
dnbin<-dist.dna(nbin, model = "K80") #computing distance by ape package with K80 model derived by Kimura (1980)
tree<-njs(dnbin) #error comes out here!!
dnbin
this is the code I used based on toparslan's script. No changes were made. Error pops out on the tree<-njs(dnbin) part