I have successfully converted a h5ad file to a h5seurat with
Convert("xxx.h5ad","h5seurat",overwrite = TRUE)
but when I tried to use the LoadH5Seurat
function from SeuratDisk R package, I got error messages like this:
Validating h5Seurat file Initializing RNA with data Error in sparseMatrix(i = x[["indices"]][] + 1, p = x[["indptr"]][], x = x[["data"]][], : 'dims' must contain all (i,j) pairs and my code is 'LoadH5Seurat("xxxx.h5seurat",assays ='RNA')'
Has anyone met the same error? Any suggestions will be really helpful!
library(SeuratDisk)
Convert("xxx.h5ad","h5seurat",overwrite = TRUE)
seuratobj <- LoadH5Seurat("xxxx.h5seurat",assays ='RNA')