Seurat UMAP visualization result is mirrored after running in two identical environments

Question

When I run the same R code in my local computer RStudio (R 4.0.2) and on Code Ocean R 4.0.3, I have two different UMAP visualization results and they are mirrored

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I use Seurat 3.2.0 version in both environments and particularly for umap visualization, here is the line:

DimPlot(MU197PDXThgFiltered, reduction = "umap", label = TRUE, label.size = 6, pt.size = 0.5)

I can't give a reproducible example, but maybe someone faced this issue before?

Answer 1

I found this on Seurat github:

The exact location of points on a UMAP plot can chance across different computers and OSs. We do our best to minimize any randomness to the procedure by fixing the random seed, but some fluctuation across systems is inevitable, and nothing to worry about

Other than point locations on UMAP, gene expressions in clusters and cell numbers in them are identical