Load fingerprint image in python array

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how to load all student fingerprints in the List by this method

for file in [file for file in os. listdir("database")]:
    fingerprint_database_image = cv2.imread("./database/"+file)

It did not load my arry if I give full path like as then ok

fingerprint_database_image = cv2.imread("d:/database/1.tif) it ok 

but I need all student's fingerprints how do I load all image please give me an idea

Waiting for your prompt help

Regard's

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