In vCard 4.0 RFC 6350 I see the following example in MEMBER property description, which is an example of vCard group:
BEGIN:VCARD
VERSION:4.0
KIND:group
FN:The Doe family
MEMBER:urn:uuid:03a0e51f-d1aa-4385-8a53-e29025acd8af
MEMBER:urn:uuid:b8767877-b4a1-4c70-9acc-505d3819e519
END:VCARD
BEGIN:VCARD
VERSION:4.0
FN:John Doe
UID:urn:uuid:03a0e51f-d1aa-4385-8a53-e29025acd8af
END:VCARD
BEGIN:VCARD
VERSION:4.0
FN:Jane Doe
UID:urn:uuid:b8767877-b4a1-4c70-9acc-505d3819e519
END:VCARD
As far as I understand both contacts as well as group in the above example are stored and served as a single vCard file (or single CardDAV request/respose).
Can a vCard file or CardDAV request/respose contain more than one vCard contact, that are not part of the group. For example like this:
BEGIN:VCARD
VERSION:4.0
FN:John Doe
END:VCARD
BEGIN:VCARD
VERSION:4.0
FN:Jane Doe
END:VCARD
?
A vCard 4 entity can contain a set of vCards, check RFC 6350 ABNF:
One or more, type doesn't matter.
But you are also asking about CardDAV. In CardDAV each vCard is always in a separate HTTP resource / a resource is never supposed to contain a stream of vCards. Check RFC 6352 Address Object Resources:
This includes groups (a vCard group in CardDAV is an own resource and doesn't contain the members).
If you want to retrieve multiple CardDAV resources in a single step, use a multiget or addressbook-query REPORT.