AWS managed Active Directory user Authentication issue

Question

My task is to connect AWS managed Active Directory from my application server

These are the steps that I followed:

1. Create an Active Directory in the us-east-1 region using AWS console

2. Launch Windows EC2 instance and connect Active Directory to that Windows instance

3. Now I install the administrative tools to manage users and computers and created one user in this Active Directory

4. I am trying to connect AWS Managed Microsoft AD to my node.js application but I get an error

    > ERROR: {"errno":-4039,
    > "code":"ETIMEDOUT",
    > "syscall":"connect", 
    > "address":"72.13.4.97","port":389}
   

Did I miss any steps to configure the Windows instance? Or do I need to add any rules in security group?

Please help me out

Thank you